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Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry
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Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry

Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry

DOI:
10.3791/50990-v

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09:40 min

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June 06, 2014

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, , , , ,
1Cancer and Blood Diseases Institute, Cincinnati Children’s Hospital Medical Center,University of Cincinnati College of Medicine, 2IBM

Chapters

  • 00:05Title
  • 01:25Ex vivo Erythroid Differentiation
  • 04:17Erythroblast Enucleation Analysis by Multi-spectral Imaging Flow Cytometry
  • 07:48Results: Representative Enucleating Erythroblast Analysis
  • 09:07Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes a novel method of identifying a population of enucleating orthochromatic erythroblasts by multi-spectral imaging flow cytometry, providing a visualization of the erythroblast enucleation process.

Tags

Keywords: ErythropoiesisEnucleationReticulocyteMulti-spectral Imaging Flow CytometryOrthochromatic ErythroblastsAspect RatioDelta CentroidTer119Draq5

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