An Improved Method for the Preparation of Type I Collagen From Skin

The overall goal of this procedure is to rapidly isolate collagen type one from a dermal sample. This is accomplished by first slicing the dermal sample into small pieces. In the second step, the non collagenous solubilized proteins and polysaccharides are removed by rapid agitation of the dermal sample in sodium acetate.

Next, the sample is rinsed with water to remove all traces of the sodium acetate. In the final step, the collagen type one is extracted from the dermal sample using citrate buffer centrifuge to remove any remaining solids from the sample and then filtered using a disposable centrifugation device to concentrate the final product. Ultimately, collagen type one can be further polymerized from this concentrate.