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An Improved Method for the Preparation of Type I Collagen From Skin
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Bioengineering
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JoVE Journal Bioengineering
An Improved Method for the Preparation of Type I Collagen From Skin

An Improved Method for the Preparation of Type I Collagen From Skin

DOI:
10.3791/51011-v

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05:17 min

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January 21, 2014

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, ,
1Department of Anesthesiology, Perioperative and Pain Medicine,Boston Children’s Hospital, 2Department of Anesthesia,Harvard Medical School

Chapters

  • 00:05Title
  • 01:10Preparing the Dermal Sample
  • 01:44Removing Noncollagenous Solubilized Material
  • 02:48Type I Collagen Extraction
  • 04:22Results: Rapid Isolation from Dermis to Yield COL1 Polymerized into Fibrils
  • 04:58Conclusion

Summary

Automatic Translation

Automatic Translation

Traditional procedures for the isolation of soluble type 1 collagen (COL1) require about 10 days from start to finish because of lengthy buffer incubations and laborious resuspensions of fibrils. Here, we describe a means to purify COL1 from small dermal biopsies in less than 3 hr.

Tags

Keywords: Type I CollagenCOL1Collagen ExtractionSkin-derived CollagenAutologous CollagenCentrifugal FiltrationRegenerative ApplicationsClinical ApplicationsCosmetic SurgeryDermal InjectionsBone GraftingReconstructive Surgery

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