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05:17 min
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January 21, 2014
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Summary
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Traditional procedures for the isolation of soluble type 1 collagen (COL1) require about 10 days from start to finish because of lengthy buffer incubations and laborious resuspensions of fibrils. Here, we describe a means to purify COL1 from small dermal biopsies in less than 3 hr.
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Cite this Article
Pacak, C. A., MacKay, A. A., Cowan, D. B. An Improved Method for the Preparation of Type I Collagen From Skin. J. Vis. Exp. (83), e51011, doi:10.3791/51011 (2014).
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