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The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry
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The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

Article DOI: 10.3791/51040-v • 10:57 min • March 11th, 2014
March 11th, 2014 •
1, 1, 1, 1, 1
1Department of Biomedical Engineering, Washington University in St. Louis

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The cut-open Vaseline gap approach is used to obtain low noise recordings of ionic and gating currents from voltage-dependent ion channels expressed in Xenopus oocytes with high resolution of fast channel kinetics. With minor modification, voltage clamp fluorometry can be coupled to the cut-open oocyte protocol.

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Xenopus Oocyte Vaseline Gap Voltage-clamp Technique Fluorometry Electrophysiological Properties Kinetic Properties Heterologous Ion Channels Cut-open Setup Two-electrode Voltage Clamp (TEVC) Technique Clamp Speed Signal-to-noise Ratio Intracellular Milieu Extracellular Milieu Human Cardiac Sodium Channel (hNaV1.5) Room Temperature Fast Activating Ion Channels Voltage Control Small Portion Of Cell Membrane Channel Run-down Patch Clamp Techniques COVG Technique Ion Channel Kinetics
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