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Ablation of a Single Cell From Eight-cell Embryos of the Amphipod Crustacean Parhyale hawaiensis
JoVE Journal
Biology
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JoVE Journal Biology
Ablation of a Single Cell From Eight-cell Embryos of the Amphipod Crustacean Parhyale hawaiensis
DOI:

10:55 min

March 16, 2014

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Chapters

  • 00:00Title
  • 00:14Introduction
  • 01:07Introduction: Animal Culture
  • 03:11Procedure: Removing a Single Cell from the 8 Cell Stage during Parhyale Development
  • 04:37Gathering Embryos: Materials
  • 06:14Ablating g: Materials
  • 07:07Ablating g: Procedure
  • 10:18Representative Results

Summary

Automatic Translation

The amphipod Parhyale hawaiensis is a promising model organism for studies of crustacean embryology and comparative arthropod development and evolution. This protocol describes a method for manual removal of single blastomeres from early cleavage stage embryos of Parhyale.

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