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Design and Analysis of Temperature Preference Behavior and its Circadian Rhythm in Drosophila
JoVE Journal
Biology
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JoVE Journal Biology
Design and Analysis of Temperature Preference Behavior and its Circadian Rhythm in Drosophila
DOI:

09:09 min

January 13, 2014

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Chapters

  • 00:05Title
  • 01:49Preparing the Flies
  • 02:35Assembling the Temperature Preference Apparatus
  • 04:06Preparing the Apparatus
  • 05:18Running the Temperature Preference Assay
  • 06:55Data Analysis
  • 07:53Circadian Rhythms and TrpA1 Affect Temperature Preference
  • 08:35Conclusion

Summary

Automatic Translation

We recently identified a novel Drosophila circadian output, temperature preference rhythm (TPR), in which the preferred temperature in flies rises during the day and falls during the night. TPR is regulated independently from another circadian output, locomotor activity. Here we describe the design and analysis of TPR in Drosophila.

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