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Chapters
- 00:05Title
- 01:38Effector CD8+ T Cell Isolation
- 03:48Target CD4+ T Cells Isolation
- 05:45Coculture
- 06:17Flow Cytometry Staining and Acquisition
- 06:58Results: Representative Flow Cytometry Cytotoxicity Assay
- 09:48Conclusion
This protocol describes a sensitive, cell-based cytotoxicity assay. By enumerating the decrease in frequency of live target CD4+ T cells in the presence of an increasing number of effector CD8+ T cells, this assay allows for the direct assessment of cytolytic activity of antigen-specific CD8+ T cells.
