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Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation
JoVE Journal
Biology
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JoVE Journal Biology
Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation

Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation

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09:07 min

March 04, 2014

DOI:

09:07 min
March 04, 2014

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Summary

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This protocol describes how to dissect premigratory cranial neural crest (NC) from Xenopus laevis neurulas. These explants can be plated on fibronectin and cultured in vitro, or grafted back into host embryos. This technique allows studying the mechanisms of NC epithelium-to-mesenchyme transition, migration, and differentiation.

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