Using Fluorescent Proteins to Visualize and Quantitate <em>Chlamydia </em>Vacuole Growth Dynamics in Living Cells

Meghan Zuck; Caroline Feng; Kevin Hybiske

doi:10.3791/51131

Journal

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Immunology and Infection

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Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Using Fluorescent Proteins to Visualize and Quantitate <em>Chlamydia </em>Vacuole Growth Dynamics in Living Cells

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

7,490 Views

•

07:42 min

•

October 13, 2015

DOI:

10.3791/51131-v

DOI:

10.3791/51131-v

•

07:42 min

October 13, 2015

•

6 Views

Meghan Zuck^1,2, Caroline Feng², Kevin Hybiske¹

¹Division of Allergy and Infectious Diseases, Department of Medicine,University of Washington, ²Program in Infectious Diseases, School of Public Health,University of California at Berkeley

Summary

A live cell fluorescent protein based method for illuminating cellular vacuoles (inclusions) containing Chlamydia is described. This strategy enables rapid, automated determination of Chlamydia infectivity in samples and can be used to quantitatively investigate inclusion growth dynamics.