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Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
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Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

DOI:
10.3791/51150-v

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12:20 min

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March 15, 2014

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, ,
1Center for Theoretical Problems of Physicochemical Pharmacology,Russian Academy of Sciences, 2Federal Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia, 3Physiology Department, Perelman School of Medicine,University of Pennsylvania

Chapters

  • 00:05Title
  • 01:21Manufacturing Reusable Flow Chambers
  • 02:55Preparing the Coverslips
  • 05:04Attaching Microtubule Seeds to the Coverslips
  • 08:04Growing Segmented Microtubules
  • 09:04Interpreting the Results
  • 11:59Conclusion

Summary

Automatic Translation

Automatic Translation

Microtubules are inherently unstable polymers, and their switching between growth and shortening is stochastic and difficult to control. Here we describe protocols using segmented microtubules with photoablatable stabilizing caps. Depolymerization of segmented microtubules can be triggered with high temporal and spatial resolution, thereby assisting analysis of motions with the disassembling microtubule ends.

Tags

Keywords: Segmented MicrotubulesMicrotubule DepolymerizationMotility AssaysProtein ComplexesMicrotubule-dependent MotionsProtein-coated BeadsFluorescence MicroscopyQuantitative Analysis

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