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Biology

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Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
 

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Article doi: 10.3791/51150
March 15th, 2014

Summary March 15th, 2014

Microtubules are inherently unstable polymers, and their switching between growth and shortening is stochastic and difficult to control. Here we describe protocols using segmented microtubules with photoablatable stabilizing caps. Depolymerization of segmented microtubules can be triggered with high temporal and spatial resolution, thereby assisting analysis of motions with the disassembling microtubule ends.

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