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Journal / Biology / Hurtig Syntese og Screening af kemisk aktiveret…
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Biology

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JoVE Journal Biology
Hurtig Syntese og Screening af kemisk aktiveret transkriptionsfaktorer med GFP-baserede Journalister
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Hurtig Syntese og Screening af kemisk aktiveret transkriptionsfaktorer med GFP-baserede Journalister

Article DOI: 10.3791/51153-v • 09:22 min • November 26th, 2013
November 26th, 2013 •
*1,3, *1, 1,2, 1
1The Lewis-Sigler Institute for Integrative Genomics, Princeton University, 2Department of Molecular Biology, Princeton University, 3Division of Chemistry and Chemical Engineering, California Institute of Technology
* These authors contributed equally

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Denne protokol beskriver en eksperimentel procedure for hurtig opbygning af kunstige transkriptionsfaktorer (ATFS) med beslægtede GFP journalister og kvantificering af ATFS evnen til at stimulere GFP udtryk via flowcytometri.

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Genetik transskription transkriptionsfaktorer kunstige transkriptionsfaktorer zink fingre Zif268 syntetisk biologi
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