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JoVE Journal
Biology

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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
 

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Article DOI: 10.3791/51153
November 26th, 2013

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Summary November 26th, 2013

This protocol describes an experimental procedure for the rapid construction of artificial transcription factors (ATFs) with cognate GFP reporters and quantification of the ATFs ability to stimulate GFP expression via flow cytometry.

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