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Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
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Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

Article DOI: 10.3791/51164-v • 10:59 min • May 19th, 2014
May 19th, 2014 •
*1,2, *1,2, 1,2
1Department of Molecular Biology, Medical Biochemistry, and Pathology, Faculty of Medicine, Laval University, 2CHU de Quebec Research Center
* These authors contributed equally

Chapters

Summary
Automatic Translation

Here, we describe a method to analyze changes in the initiation of mRNA translation of eukaryotic cells in response to stress conditions. This method is based on the velocity separation on sucrose gradients of translating ribosomes from non-translating ribosomes.

Tags

Translation Initiation Stress Conditions Polysome Profiling MRNA Translation Control Molecular Basis Density Gradient Fractionation System Translational Control Polysome Analysis Ultracentrifugation Sucrose Gradient Translating Ribosomes Populations Polysome Profile Translation Initiation Defects Specific Proteins Role Translational Activity Eukaryotic Cells And Tissues
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