Here, we describe a method to analyze changes in the initiation of mRNA translation of eukaryotic cells in response to stress conditions. This method is based on the velocity separation on sucrose gradients of translating ribosomes from non-translating ribosomes.
Coudert, L., Adjibade, P., Mazroui, R. Analysis of Translation Initiation During Stress Conditions by Polysome Profiling. J. Vis. Exp. (87), e51164, doi:10.3791/51164 (2014).