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Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
JoVE Journal
Biology
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JoVE Journal Biology
Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

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10:59 min

May 19, 2014

DOI:

10:59 min
May 19, 2014

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Summary

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Here, we describe a method to analyze changes in the initiation of mRNA translation of eukaryotic cells in response to stress conditions. This method is based on the velocity separation on sucrose gradients of translating ribosomes from non-translating ribosomes.

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