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Biology

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Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
 

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

Article DOI: 10.3791/51164
May 19th, 2014

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Summary May 19th, 2014

Here, we describe a method to analyze changes in the initiation of mRNA translation of eukaryotic cells in response to stress conditions. This method is based on the velocity separation on sucrose gradients of translating ribosomes from non-translating ribosomes.

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