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JoVE Journal Chemistry
Isolering og fremstilling av bakterielle cellevegger for komposisjonsanalyse ved ultraytelses væskekromatografi
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Isolering og fremstilling av bakterielle cellevegger for komposisjonsanalyse ved ultraytelses væskekromatografi

Article DOI: 10.3791/51183-v • 11:18 min • January 15th, 2014
January 15th, 2014 •
1, 2, 3, 1,4
1Department of Bioengineering, Stanford University, 2Department of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden, Umeå Centre for Microbial Research, Umeå University, 3Campus de Cantoblanco, Universidad Autonoma de Madrid, 4Department of Microbiology and Immunology, Stanford University School of Medicine

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Den bakterielle celleveggen består av peptidoglykan, et makromolekylært nettverk av sukkerstrenger krysskoblet av peptider. Ultra Ytelse Flytende kromatografi gir høy oppløsning og gjennomstrømning for nye funn av peptidoglykan sammensetning. Vi presenterer en prosedyre for isolering av cellevegger (sacculi) og deres påfølgende forberedelse til analyse via UPLC.

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Kjemi Utgave 83 peptidoglykan bakteriell cellevegg ultraytelses væskekromatografi høyytelses væskekromatografi celleform morfogenese
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