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Immunology and Infection

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Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

Article DOI: 10.3791/51189-v • 13:34 min • March 17th, 2014
March 17th, 2014 •
1, 1,2
1Department of Immunology, The University of Texas MD Anderson Cancer Center, 2The University of Texas Graduate School of Biomedical Sciences

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Summary
Automatic Translation

This protocol describes experimental procedures to assess the differentiation of plasmacytoid dendritic cells in Peyer’s patch from common dendritic cell progenitors, using techniques involving FACS-mediated cell isolation, hydrodynamic gene transfer, and flow analysis of immune subsets in Peyer’s patch.

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Murine Plasmacytoid Dendritic Cells Peyer's Patches Adoptive Transfer Hematopoietic Progenitors Dendritic Cell Progenitors CDPs Flt3 Ligand Flt3L Hydrodynamic Gene Transfer CD45 Marker Expression Flow Cytometry Single Cell Suspensions
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