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JoVE Journal
Biology

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Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana
 

Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana

Article doi: 10.3791/51204
April 19th, 2014

Summary April 19th, 2014

Transient protein production in Nicotiana plants based on vacuum infiltration with Agrobacteria carrying launch vectors (Tobacco mosaic virus-based) is a rapid and economic approach to produce vaccine antigens and therapeutic proteins. We simplified the procedure and improved target accumulation by optimizing conditions of bacteria cultivation, selecting host species, and co-introducing RNA silencing suppressors.

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