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The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
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JoVE Journal Biology
The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

DOI:
10.3791/51220-v

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24:02 min

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April 11, 2014

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,
1Department of Experimental Oncology,European Institute of Oncology

Chapters

  • 00:05Title
  • 02:59Native Chromatin Immunoprecipitation (N-ChIP)
  • 08:24Crosslinking Chromatin Immunoprecipitation (X-ChIP)
  • 12:05Sample Preparation Prior to MS
  • 14:50Results: ChroP Enables Proteomic Characterization of Chromatin Composition at Distinct Functional Regions
  • 23:19Conclusion

Summary

Automatic Translation

Automatic Translation

By combining native and crosslinking chromatin immunoprecipitation with high-resolution Mass Spectrometry, ChroP approach enables to dissect the composite proteomic architecture of histone modifications, variants and non-histonic proteins synergizing at functionally distinct chromatin domains.

Tags

Keywords: ChromatinChIPMass SpectrometryProteomicsHistone Post-translational ModificationsChromatin-binding ProteinsTranscription FactorsDNA MethylationHeterochromatinHistone H3 Tri-methylation

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