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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

DOI:
10.3791/51234-v

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12:02 min

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April 18, 2014

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, , ,
1Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering,Harvard University, 2Department of Systems Biology,Harvard Medical School, 3Department of Biotechnology,Delft University of Technology

Chapters

  • 00:05Title
  • 01:11Design of Sequences for Transient Expression
  • 02:06Gibson Assembly of DNA Parts
  • 06:16Biolistic Transfection of Tobacco with the Gene Gun
  • 08:50Representative Images of Biolistically Transfected Tobacco Leaf Cells
  • 11:18Conclusion

Summary

Automatic Translation

Automatic Translation

This work describes a novel method for selectively targeting subcellular organelles in plants, assayed using the BioRad Gene Gun.

Tags

Keywords: Alternative SplicingChloroplastCytosolGibson AssemblyGFPGene GunMetabolic EngineeringMulti-organelle TargetingPeroxisomeSynthetic BiologyTobaccoTransient Gene Expression

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