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An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings
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JoVE Journal Medicine
An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

DOI:
10.3791/51242-v

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19:57 min

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March 30, 2014

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, , , , , , , , , , ,
1Africa Centre for Health and Population Studies, College of Health Sciences,University of KwaZulu-Natal, Durban, South Africa, 2Unit D11,Jembi Health Systems, 3Academic Medical Center, Department of Global Health, Amsterdam Institute for Global Health and Development (AIGHD),University of Amsterdam, 4Division of Infectious Diseases and Geographic Medicine, Centre for AIDS Research,Stanford Medical School

Chapters

  • 00:00Title
  • 00:48RNA Extraction
  • 02:39Reverse Transcription
  • 06:06PCR Purification
  • 07:39Sequencing
  • 11:11Bioinformatics
  • 18:40Conclusion

Summary

Automatic Translation

Automatic Translation

Drug resistance testing for HIV-1 infected individuals failing antiretroviral therapy (ART) can guide future therapies and improve treatment outcomes. Optimizing individual and population health outcomes in high HIV prevalence but resource-limited settings will ultimately require affordable and accessible drug resistance genotyping and interpretation methods.

Tags

Keywords: HIV-1Drug ResistanceAntiretroviral TherapyGenotypingResource-limited SettingsOpen-source SoftwareViral LoadCRF02_AGSubtypes A And B

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