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A Mouse Tumor Model of Surgical Stress to Explore the Mechanisms of Postoperative Immunosuppression and Evaluate Novel Perioperative Immunotherapies
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
A Mouse Tumor Model of Surgical Stress to Explore the Mechanisms of Postoperative Immunosuppression and Evaluate Novel Perioperative Immunotherapies
DOI:

13:37 min

March 12, 2014

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Chapters

  • 00:05Title
  • 01:41Culturing and Harvesting 4T1 Tumor Cells
  • 03:18Injecting Mice with 4T1 Tumor Cells
  • 04:39Administrating Perioperative Treatment in 4T1 Tumor Bearing BALB/c Mice
  • 06:03Complete Resection of Primary Tumor and Abdominal Left Nephrectomy
  • 08:23Lung Removal and Quantification of Lung Tumor Burden
  • 09:32Results: Lung Metastases, Surgical Stress, and Perioperative Therapy
  • 12:53Conclusion

Summary

Automatic Translation

A mouse tumor model of surgical stress is used to explore how postoperative immune suppression promotes metastatic disease and to evaluate immunostimulatory perioperative therapies.

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