Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

Jaimy Miller; Daniela Dalm; Alexey Y. Koyfman; Kirill Grushin; Svetla Stoilova-McPhie

doi:10.3791/51254

Journal

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Bioengineering

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Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

12,224 Views

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12:24 min

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June 03, 2014

DOI:

10.3791/51254-v

DOI:

10.3791/51254-v

•

12:24 min

June 03, 2014

•

2 Views

Jaimy Miller*¹, Daniela Dalm*¹, Alexey Y. Koyfman^2,3, Kirill Grushin¹, Svetla Stoilova-McPhie^1,3

¹Department of Neuroscience and Cell Biology,University of Texas Medical Branch, ²Department of Biochemistry and Molecular Biology,University of Texas Medical Branch, ³Sealy Center for Structural Biology and Molecular Biophysics,University of Texas Medical Branch

Summary

We present a combination of Cryo-electron microscopy, lipid nanotechnology, and structure analysis applied to resolve the membrane-bound structure of two highly homologous FVIII forms: human and porcine. The methodology developed in our laboratory to helically organize the two functional recombinant FVIII forms on negatively charged lipid nanotubes (LNT) is described.