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Biology

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Assaying Proteasomal Degradation in a Cell-free System in Plants
 

Assaying Proteasomal Degradation in a Cell-free System in Plants

Article DOI: 10.3791/51293
March 26th, 2014

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Summary March 26th, 2014

Targeted protein degradation represents a major regulatory mechanism for cell function. It occurs via a conserved ubiquitin-proteasome pathway, which attaches polyubiquitin chains to the target protein that then serve as molecular “tags” for the 26S proteasome. Here, we describe a simple and reliable cell-free assay for proteasomal degradation of proteins.

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