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Journal / Immunology and Infection / Bioenergetics and the Oxidative Burst: Protocols…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Bioenergetics and the Oxidative Burst
Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
Journal (Immunology and Infection)
Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
Journal (Immunology and Infection)
"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)
 

Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets

Article DOI: 10.3791/51301-v • 11:40 min • March 27th, 2014
March 27th, 2014 •
*1, *1, 1, 1, 1, *1
1UAB Mitochondrial Medicine Laboratory, Center for Free Radical Biology, Department of Pathology, University of Alabama at Birmingham
* These authors contributed equally

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Summary
Automatic Translation

Blood leukocytes and platelets can be used as a marker of overall bioenergetic health of an individual and so have the potential to monitor pathological processes and the impact of treatments. Here we describe a method to isolate and measure mitochondrial function and the oxidative burst in these cells.

Tags

Bioenergetics Oxidative Burst Isolation Evaluation Human Leukocytes Platelets Mitochondrial Dysfunction Pathological Conditions Atherosclerosis Diabetes Septic Shock Neurodegenerative Diseases Bioenergetic Function Patients Hyperglycemia Inflammation Circulating Leukocytes Platelets Magnetic Bead Isolation Techniques Cell Adhesion Techniques High Throughput Microplate Respirometry Extracellular Flux Analyzer
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