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The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics
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Chapters
- 00:05Title
- 01:29Preparing the Thin Lipid Film
- 02:57Lipid Resuspension and Vesicle Homogenization
- 04:00Vesicle Extrusion and Lyophilization
- 06:38Encapsulating Transcription-translation Machinery
- 08:14Microscopy
- 08:52Results: Fluorescent Microscopy Images
- 09:54Conclusion
Herein we describe simple methods for the preparation of vesicles, the encapsulation of transcription and translation machinery, and the monitoring of protein production. The resulting cell-free systems can be used as a starting point from which to build increasingly complex cellular mimics.
