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JoVE Journal
Biology

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Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging
 

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging

Article doi: 10.3791/51366
January 2nd, 2014 Usage Statistics

Summary January 2nd, 2014

The attachment of virions to a surface is a requirement for single virion imaging by Super-resolution fluorescence imaging or atomic force microscopy (AFM). Here we demonstrate a sample preparation method for controlled adhesion of virions to glass surfaces suitable for use in AFM and super-resolution fluorescence imaging.

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