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Biology

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An in vivo Crosslinking Approach to Isolate Protein Complexes From Drosophila Embryos
 

An in vivo Crosslinking Approach to Isolate Protein Complexes From Drosophila Embryos

Article DOI: 10.3791/51387
April 23rd, 2014

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Summary April 23rd, 2014

Multi-component protein complexes play crucial roles during cellular function and development. Here we describe a method used to isolate native protein complexes from Drosophila embryos after in vivo crosslinking followed by purification of the crosslinked complexes for subsequent structure-function analysis.

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