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Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
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JoVE Journal Biology
Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy

DOI:
10.3791/51395-v

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09:46 min

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April 28, 2014

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, , , ,
1Department of Pediatrics, Faculty of Medicine and Health Sciences,Université de Sherbrooke, 2Biophotonics Core Facility,Centre de Recherche Clinique Etienne-Le Bel

Chapters

  • 00:05Title
  • 01:41Preparing Solutions and Cells
  • 03:58Imaging the Cells
  • 05:53Data Analysis
  • 07:03Intracellular pH Changes in HT-1080 Cells
  • 08:57Conclusion

Summary

Automatic Translation

Automatic Translation

Several methods are available for measuring intracellular pH but very few of these allow simultaneous measurement of cytoplasmic and organellar pH. Here, we describe in detail a rapid and accurate methodology to simultaneously measure cytoplasmic and vesicular pH by ratiometric imaging of living cells.

Tags

Keywords: PH MeasurementIntracellular PHCytoplasmOrganellesEndocytic CompartmentsConfocal MicroscopySNARF-1HPTSRatiometric DyesPH CalibrationPH HomeostasisVesicular TraffickingEndosomal-lysosomal Pathway

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