Journal
/
Biology
/
Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

DOI:
10.3791/51420-v

•

14:53 min

•

May 13, 2014

•

, , , , ,
1Centre for Gene Regulation and Expression, College of Life Sciences,University of Dundee, UK

Chapters

  • 00:05Title
  • 02:14Cell Culture and Treatment
  • 04:58Click-iT Assay
  • 09:59Light Microscopy and Data Analysis
  • 12:41Results: RNA Expression in Hypoxic Cells
  • 14:02Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a technique for analysis of global RNA synthesis in hypoxia using imaging. Click-chemistry labeling of RNA has not previously been performed under hypoxia and allows visualization of global RNA changes at the single cell level. This approach complements the existing averaged RNA techniques, allowing direct visualization of cell-to-cell changes in global RNA synthesis.

Tags

Keywords: Single Cell AnalysisRNA SynthesisHypoxiaOxygen SensingTranscriptional ResponseCancer CellsMicroscopy AnalysisClick-iT RNA Imaging

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51420?article_type=v

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
check_url/51420?article_type=v

Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure
check_url/51420?article_type=v

Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)
check_url/51420?article_type=v

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
check_url/51420?article_type=v

Measurement of Heme Synthesis Levels in Mammalian Cells
check_url/51420?article_type=v

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
check_url/51420?article_type=v

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
check_url/51420?article_type=v

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
check_url/51420?article_type=v

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations
check_url/51420?article_type=v

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

Read Article