Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

This content is Open Access.

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
 

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Article DOI: 10.3791/51420
May 13th, 2014

Chapters

Summary May 13th, 2014

We describe a technique for analysis of global RNA synthesis in hypoxia using imaging. Click-chemistry labeling of RNA has not previously been performed under hypoxia and allows visualization of global RNA changes at the single cell level. This approach complements the existing averaged RNA techniques, allowing direct visualization of cell-to-cell changes in global RNA synthesis.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter