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Single Wavelength Shadow Imaging of Caenorhabditis elegans Locomotion Including Force Estimates
JoVE Journal
Engineering
This content is Free Access.
JoVE Journal Engineering
Single Wavelength Shadow Imaging of Caenorhabditis elegans Locomotion Including Force Estimates
DOI:

08:41 min

April 18, 2014

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Chapters

  • 00:05Title
  • 01:34Experimental Set Up
  • 02:53Recording C. elegans Movement
  • 03:48Data Analysis
  • 05:39Thrust Against Gravity
  • 07:35Conclusion

Summary

Automatic Translation

The technique presented here measures the path of freely swimming microscopic species using single wavelength exposure. C. elegans are used to demonstrate shadow imaging as an inexpensive alternative to costly microscopes. This technique can be adapted to accommodate various orientations, environments and species to measure direction, speed, acceleration and forces.

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