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JoVE Journal Biology
Rekonstituering af β-catenin nedbrydning i
 
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Rekonstituering af β-catenin nedbrydning i

Article doi: 10.3791/51425
June 17th, 2014 •
*1, *1, 2, 1,3
1Department of Cell and Developmental Biology and Program in Developmental Biology, Vanderbilt University Medical Center, 2Division of Gastroenterology, Hepatology & Nutrition and Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 3Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine
* These authors contributed equally

Summary
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June 17th, 2014

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Der beskrives en fremgangsmåde til analyse af protein-nedbrydning under anvendelse af radioaktivt mærket og luciferase-fusionsproteiner i Xenopus æggeekstrakt og dens tilpasning til high-throughput screening for små molekyler modulatorer af proteinnedbrydning.

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