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JoVE Journal Neuroscience
Lineage-reprogramação de células pericyte derivados do Adulto Cérebro Humano em neurônios induzidos
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Lineage-reprogramação de células pericyte derivados do Adulto Cérebro Humano em neurônios induzidos

Article DOI: 10.3791/51433-v • 09:36 min • May 12th, 2014
May 12th, 2014 •
1, 2, 3, 1,4
1Department of Physiological Genomics, Institute of Physiology, Ludwig Maximilians University Munich, 2Tumor Biology Lab, Neurosurgical Clinic, Ludwig-Maximilians University Munich, 3Institut für Biochemie, Emil-Fischer-Zentrum, Friedrich-Alexander-Universität Erlangen-Nürnberg, 4Institute of Physiological Chemistry and Focus Program Translational Neuroscience, Johannes Gutenberg University Mainz

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Segmentação células cerebrais residente linhagem-reprogramação direta oferece novas perspectivas de reparação do cérebro. Aqui nós descrevemos um protocolo de como preparar culturas enriquecido para pericitos cerebrais residente do córtex cerebral humano adulto e convertê-las em neurônios induzidos pela expressão mediada por retrovírus de fatores de transcrição Sox2 e Ascl1.

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Neurociência Edição 87 Pericitos linhagem-reprogramação os neurônios induzidos córtex cerebral
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