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MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules
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MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

DOI:
10.3791/51434-v

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09:59 min

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March 05, 2014

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,
1Department of Chemistry,University of Wisconsin- Madison, 2School of Pharmacy,University of Wisconsin- Madison

Chapters

  • 00:05Title
  • 01:12Tissue Preparation
  • 02:27Matrix Application
  • 04:28Image Acquisition, Image Generation, and Metabolite Identification
  • 06:28Results: Discovery and Identification of Small Molecule Analytes in Intact Tissue by MALDI-MSI
  • 09:28Conclusion

Summary

Automatic Translation

Automatic Translation

Mass spectrometric imaging (MSI) is a powerful tool that can be used to discover and identify various chemical species in intact tissues, preserving the compounds in their native environments, which can provide new insights into biological processes. Herein a MSI method developed for the analysis of small molecules is described. 

Tags

Keywords: MALDI-MS ImagingMetabolomicsMedicago TruncatulaRoot NodulesSmall MoleculesSpatial DistributionMatrix Application

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