Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Investigating Protein-protein Interactions in…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
Installing CLOCCS and Using it to Parameterize the Experiments
Journal (Biology)
Installing CLOCCS and Using it to Parameterize the Experiments
Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
Journal (Biology)
Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
 

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

Article DOI: 10.3791/51438 • 11:46 min • May 26th, 2014
May 26th, 2014 •
*1, *1, 1, 1,2
1Language and Genetics Department, Max Planck Institute for Psycholinguistics, 2Donders Institute for Brain, Cognition and Behaviour
* These authors contributed equally

Chapters

Summary
Automatic Translation

Interactions between proteins are fundamental to all cellular processes. Using Bioluminescence Resonance Energy Transfer, the interaction between a pair of proteins can be monitored in live cells and in real time. Furthermore, the effects of potentially pathogenic mutations can be assessed.

Tags

Protein-protein Interactions Live Cells Bioluminescence Resonance Energy Transfer BRET Assay Donor Luciferase Enzyme Acceptor Fluorescent Protein Renilla Reniformis Luciferase Yellow Fluorescent Protein (YFP) Distance-dependent Energy Transfer Cultured Mammalian Cells Fusion Proteins Weak Interactions Transient Interactions Biochemical Environment Yeast Two-hybrid Mass Spectrometry Proteomics Studies Mapping Interacting Regions
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter