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Chapters
- 00:05Title
- 01:52Isolation of Dry Algal Biomass to be Used as Standards for Fluorescence Readings
- 02:52Gravimetric Quantification of Neutral Lipids by Hexane Extraction
- 04:48Fluorometric Quantification of Neutral Lipids Using Nile Red
- 06:42Fluorescence Microscopy Technique
- 08:04Results: Images and Calibration Curves of A. protothecoides Stained with Nile Red
- 10:18Conclusion
En simpel protokol til at bestemme det neutrale lipid indholdet af algeceller hjælp af en nilrødt farvning fremgangsmåde er beskrevet. Denne tidsbesparende teknik giver et alternativ til traditionelle gravimetriske-baserede lipid kvantificering protokoller. Det har været designet til den konkrete anvendelse af overvågning bioproces ydeevne.
