Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolamento de ARN a partir de pequenas…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Isolamento de ARN a partir de pequenas não-codificante de Soro Humano
Isolation and Purification of Kinesin from Drosophila Embryos
Journal (Biology)
Isolation and Purification of Kinesin from Drosophila Embryos
Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes
Journal (Biology)
Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes
 
Click here for the English version

Isolamento de ARN a partir de pequenas não-codificante de Soro Humano

Article DOI: 10.3791/51443-v • 06:44 min • June 19th, 2014
June 19th, 2014 •
1, 1, 2,3
1School of Medical and Molecular Biosciences, Faculty of Science, University of Technology, Sydney, 2Centre for Health Technologies, Faculty of Engineering and Information Technology, University of Technology, Sydney, 3The Sydney Head and Neck Cancer Institute, Sydney Cancer Centre, Royal Prince Alfred Hospital

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Este protocolo descreve um método para extrair pequenos RNAs de soro humano. Temos utilizado este método para isolar microRNAs de soro câncer para uso em matrizes de DNA e também singleplex PCR quantitativo. O protocolo utiliza fenol e tiocianato de guanidina reagentes com modificações para produzir RNA de alta qualidade.

Tags

Bioengenharia pequeno isolamento não codificante RNA os microRNAs soro humano qPCR guanidina tiocianato Phase Lock Gel matrizes
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter