This content is Free Access.
Chapters
- 00:05Title
- 02:04Sample Preparation
- 04:05Preparation of the Microscope
- 05:01Image Acquisition
- 06:44Checking Data Quality and Reconstructing 3D-SIM Images
- 08:01Generating 3D Intensity Plots of the Z Ring from 3D-SIM Image Data
- 09:58Results: OMX Fast 3D-SIM to Investigate FtsZ-GFP Localization and Dynamics in Live Bacillus subtilis
- 11:39Conclusion
Spatiotemporal information about dynamic proteins inside live cells is crucial for understanding biology. A type of super-resolution microscopy called fast 3D-structured illumination microscopy (f3D-SIM) reveals unique information about the cytokinetic Z ring in bacteria: both its bead-like appearance and the rapid dynamics of FtsZ within the ring.
