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Chemistry

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Hot Biological Catalysis
 

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Article doi: 10.3791/51487
April 4th, 2014

Summary April 4th, 2014

Isothermal titration calorimetry measures heat flow released or absorbed in chemical reactions. This method can be used to quantify enzyme-catalysis. In this paper, the protocol for instrumental setup, experiment running, and data analysis is generally described, and applied to the characterization of enzymatic urea hydrolysis by jack bean urease.

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