Chemistry
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Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
Chapters
Summary April 4th, 2014
Isothermal titration calorimetry measures heat flow released or absorbed in chemical reactions. This method can be used to quantify enzyme-catalysis. In this paper, the protocol for instrumental setup, experiment running, and data analysis is generally described, and applied to the characterization of enzymatic urea hydrolysis by jack bean urease.
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