Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Christopher W. Coyne; Karan Patel; Johanna Heureaux; Jeanne Stachowiak; Daniel A. Fletcher; Allen P. Liu

doi:10.3791/51510

Journal

/

Bioengineering

/

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

14,761 Views

•

08:35 min

•

February 21, 2014

DOI:

10.3791/51510-v

DOI:

10.3791/51510-v

•

08:35 min

February 21, 2014

•

11 Views

Christopher W. Coyne¹, Karan Patel¹, Johanna Heureaux¹, Jeanne Stachowiak³, Daniel A. Fletcher^4,5, Allen P. Liu^1,2

¹Department of Mechanical Engineering,University of Michigan, ²Department of Biomedical Engineering,University of Michigan, ³Department of Biomedical Engineering, Institute for Cellular and Molecular Biology,The University of Texas at Austin, ⁴Department of Bioengineering,University of California, Berkeley, ⁵Physical Biosciences Division,Lawrence Berkeley National Laboratory

Summary

Microfluidic jetting against a droplet interface lipid bilayer provides a reliable way to generate vesicles with control over membrane asymmetry, incorporation of transmembrane proteins, and encapsulation of material. This technique can be applied to study a variety of biological systems where compartmentalized biomolecules are desired.