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Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
JoVE Journal
Medicine
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JoVE Journal Medicine
Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
DOI:

09:15 min

May 23, 2014

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Chapters

  • 00:05Title
  • 01:34Prepariation of Primary Cell Lines using the Scraping Method
  • 04:56Preparation of Primary Cell Lines using the Trypsin Method
  • 07:07Results: Establishment of an Endometrial Stromal Culture
  • 08:53Conclusion

Summary

Automatic Translation

Establishing primary endometrial stromal cell culture systems from hysterectomy specimens is a valuable biological technique and a crucial step prior to pursuing a vast array of research aims. Here, we describe two methods used to establish stromal cultures from surgically resected endometrial tissues of human patients. 

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