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Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
 

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Article DOI: 10.3791/51519
July 24th, 2014

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Summary July 24th, 2014

Here, we present human pluripotent stem cell (hPSC) culture protocols, based on non-colony type monolayer (NCM) growth of dissociated single cells. This new method, utilizing Rho-associated kinase inhibitors or the laminin isoform 521 (LN-521), is suitable for producing large amounts of homogeneous hPSCs, genetic manipulation, and drug discovery.

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