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JoVE Journal Developmental Biology
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Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Article DOI: 10.3791/51526 • 14:50 min • January 12th, 2015
January 12th, 2015 •
1, 2,3, 4, 2,5, 2,4,5
1Developmental and Stem Cell Biology, Hospital for Sick Children, 2Children's Health Research Institute, University of Western Ontario, 3Department of Physiology and Pharmacology, University of Western Ontario, 4Neurosciences and Mental Health, Hospital for Sick Children, 5Department of Paediatrics, University of Western Ontario

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Summary
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The Xenopus laevis embryo continues to be exceptionally useful in the study of early development due to its large size and ease of manipulation. A simplified protocol for whole mount in situ hybridization protocol is provided that can be used in the identification of specific organs in this model system.

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Organogenesis In Situ Hybridization Xenopus Laevis Gene Expression RNA Probes Organ Identification Gene Manipulation Signaling Pathways Simplified Protocol Reagents In Situ Hybridization Robots Imaging Optimization Xenopus Tropicalis
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