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Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus
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JoVE Journal Developmental Biology
Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

DOI:
10.3791/51526-v

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14:50 min

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January 12, 2015

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, , , ,
1Developmental and Stem Cell Biology,Hospital for Sick Children, 2Children’s Health Research Institute,University of Western Ontario, 3Department of Physiology and Pharmacology,University of Western Ontario, 4Neurosciences and Mental Health,Hospital for Sick Children, 5Department of Paediatrics,University of Western Ontario

Chapters

  • 00:05Title
  • 01:52Embryo Preparation
  • 03:45Probe Preparation
  • 06:49In Situ Hybridization
  • 10:49Imaging Embryos
  • 12:38Results: A Simplified In Situ Hybridization Protocols Identifies Specific Organs in Xenopus Embryos
  • 14:26Conclusion

Summary

Automatic Translation

Automatic Translation

The Xenopus laevis embryo continues to be exceptionally useful in the study of early development due to its large size and ease of manipulation. A simplified protocol for whole mount in situ hybridization protocol is provided that can be used in the identification of specific organs in this model system.

Tags

Keywords: XenopusOrganogenesisIn Situ HybridizationRNA ProbesImagingGene ExpressionOrgan DevelopmentSimplified ProtocolIn Situ Hybridization Robots

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