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Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos

JoVE Journal
Biology

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JoVE Journal Biology

Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos

Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos

DOI:

10.3791/51533-v

•

07:06 min

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May 17, 2014

•

Shannon N. Romano, Daniel A. Gorelick

¹Department of Pharmacology and Toxicology,University of Alabama at Birmingham

Chapters

00:04Title
01:15Chemical Treatment of Embryos
03:05Automated Imaging of Embryos in 96-well Plates
05:21Results: Semi-automated, Tissue-specific Imaging
06:35Conclusion

Summary

Automatic Translation

Described here is a protocol for semi-automated imaging of tissue-specific fluorescence in zebrafish embryos.

Tags

Keywords: Zebrafish Embryos Fluorescent Reporter Chemical Screening Estrogen Receptor Automated Imaging Epifluorescence Microscope Transgenic Zebrafish High-throughput Screening Gene Expression

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