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A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

DOI:
10.3791/51549-v

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07:04 min

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May 23, 2014

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, , , , , , , , ,
1Plate-Forme d'Imagerie Dynamique, Imagopole,Institut Pasteur, 2Department of Radiation Oncology,Stanford School of Medicine, 3Service Hospitalier Frédéric Joliot,Institut d'Imagerie Biomédicale, 4Vanderbilt School of Medicine, 5The Walter & Eliza Hall Institute of Medical Research, 6Unité INSERM U786,Institut Pasteur, 7Unité de Pathogénie Microbienne Moléculaire,Institut Pasteur

Chapters

  • 00:05Title
  • 01:09Basic Recapitulation of FUEL
  • 02:32FUEL Over Varying Distances
  • 04:49Investigating Potential FUEL Pairs
  • 05:53Results: Analysis of the Various FUEL Demonstrations
  • 06:40Conclusion

Summary

Automatic Translation

Automatic Translation

Expanding the foundation and applicability of Fluorescence by Unbound Excitation from Luminescence (FUEL) by surveying the relevant principles and demonstrating its compatibility with a multitude of fluorophores and antibody-targeted conditions.

Tags

Keywords: FUELFluorescenceLuminescenceBRETRadiative ExcitationEnergy TransferFluorophoresFluorescent NanoparticlesAntibody-targeted FUELIn VitroIn VivoWhole Animal Imaging

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