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Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo
 

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

Article DOI: 10.3791/51593
September 18th, 2014

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Summary September 18th, 2014

The aim of the protocol is to use optimal methods for stimulating the cytoplasmic DNA sensing pathways in cells and in vivo. This is achieved by improving the generation of long, double-stranded DNA during blunt-end ligation. Cells or mice are then transfected using a lipid transfection reagent.

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