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EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
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EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

DOI:
10.3791/51611-v

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06:01 min

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November 26, 2014

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, ,
1Department of Biotechnology,Delft University of Technology

Chapters

  • 00:05Title
  • 01:07Preparation of Setup
  • 02:06Perform Redox Titration
  • 03:12Measure Titration Samples Using EPR Spectroscopy and Data Analysis
  • 04:12Results: Identification of Redox Active Cofactors of S. cerevisiae Nar1 and Their Midpoint Potentials
  • 05:33Conclusions

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to use electron paramagnetic resonance (EPR) monitored redox titrations to identify different cofactors of Saccharomyces cerevisiae Nar1. Redox titrations offer a very robust way to obtain midpoint potentials of different redox active cofactors in enzymes and proteins.

Tags

Keywords: EPRRedox TitrationSaccharomyces Cerevisiae Nar1CofactorsElectron Paramagnetic ResonanceMidpoint PotentialVoltammetryRedox MediatorsNernst Equation

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