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EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
 

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

Article DOI: 10.3791/51611
November 26th, 2014

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Summary November 26th, 2014

The goal of this protocol is to use electron paramagnetic resonance (EPR) monitored redox titrations to identify different cofactors of Saccharomyces cerevisiae Nar1. Redox titrations offer a very robust way to obtain midpoint potentials of different redox active cofactors in enzymes and proteins.

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