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Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

DOI:
10.3791/51646-v

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09:14 min

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June 13, 2014

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, , ,
1Department of Medicine,New York University School of Medicine, 2Departments of Pathology,New York University School of Medicine

Chapters

  • 00:05Title
  • 01:27Microplate and T Cell Preparation
  • 03:57Cell Stimulation
  • 05:43Percent Adherent Cells Analysis
  • 07:11Results: Representative Stimulated T Cell Data
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

Static adhesion assay is a powerful tool that can be used to model the interactions between T lymphocytes and other cell types. Interactions are generated by injecting labeled T cells into wells coated with adhesion molecules, while a plate reader is used to quantify the number of adherent cells following serial washes.

Tags

Keywords: T Lymphocyte AdhesionIntegrin ActivationLFA-1ICAM-1Static Adhesion AssayInside-out SignalingCell AdhesionCell MigrationImmunological SynapseVLA-4Fibronectin

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