Measuring the Osmotic Water Permeability Coefficient (P<sub>f</sub>) of Spherical Cells: Isolated Plant Protoplasts as an Example

Arava Shatil-Cohen; Hadas Sibony; Xavier Draye; Fran&#231;ois Chaumont; Nava Moran; Menachem Moshelion

doi:10.3791/51652

Journal

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Biology

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Measuring the Osmotic Water Permeability Coefficient (P_f) of Spherical Cells: Isolated Plant Protoplasts as an Example

Measuring the Osmotic Water Permeability Coefficient (P<sub>f</sub>) of Spherical Cells: Isolated Plant Protoplasts as an Example

JoVE Journal
Biology

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JoVE Journal Biology

Measuring the Osmotic Water Permeability Coefficient (P_f) of Spherical Cells: Isolated Plant Protoplasts as an Example

15,980 Views

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14:20 min

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October 08, 2014

DOI:

10.3791/51652-v

DOI:

10.3791/51652-v

•

14:20 min

October 08, 2014

•

2 Views

Arava Shatil-Cohen¹, Hadas Sibony¹, Xavier Draye², François Chaumont³, Nava Moran¹, Menachem Moshelion¹

¹The RH Smith Institute of Plant Sciences and Genetics in Agriculture,The Hebrew University of Jerusalem, ²Earth and Life Institute,Université catholique de Louvain, ³Institut des Sciences de la Vie,Université catholique de Louvain

Summary

Measuring the osmotic water permeability coefficient (P_f) of cells can help understand the regulatory mechanisms of aquaporins (AQPs). P_f determination in spherical plant cell protoplasts presented here involves protoplasts isolation and numerical analysis of their initial rate of volume change as a result of an osmotic challenge during constant bath perfusion.