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Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example
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Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example

Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example

DOI:
10.3791/51652-v

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14:20 min

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October 08, 2014

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, , , , ,
1The RH Smith Institute of Plant Sciences and Genetics in Agriculture,The Hebrew University of Jerusalem, 2Earth and Life Institute,Université catholique de Louvain, 3Institut des Sciences de la Vie,Université catholique de Louvain

Chapters

  • 00:05Title
  • 02:10Isolation of Arabidopsis Mesophyll Protoplasts
  • 04:13The Hypotonic Challenge Assay: Arabidopsis Mesophyll Cell Swelling
  • 06:36Analysis of Cell Volume Change Using ImageJ
  • 08:12Modling the Rate of Osmolarity Change in the Experimental Chamber Using ImageJ and the Matlab Program PfFit
  • 11:11Determining the Pf Using the Matlab Fitting Program PfFit
  • 12:30Results: Determination of the Osmotic Water Permeability Coefficient (Pf) in Plant Protoplasts
  • 13:37Conclusion

Summary

Automatic Translation

Automatic Translation

Measuring the osmotic water permeability coefficient (Pf) of cells can help understand the regulatory mechanisms of aquaporins (AQPs). Pf determination in spherical plant cell protoplasts presented here involves protoplasts isolation and numerical analysis of their initial rate of volume change as a result of an osmotic challenge during constant bath perfusion.

Tags

Osmotic Water PermeabilityPlant ProtoplastsAQP RegulationCell Volume ChangesHypotonic SolutionOsmotic Challenge AssayPf Measurement

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