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Glutamine Flux Imaging Using Genetically Encoded Sensors
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Bioengineering
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JoVE Journal Bioengineering
Glutamine Flux Imaging Using Genetically Encoded Sensors

Glutamine Flux Imaging Using Genetically Encoded Sensors

DOI:
10.3791/51657-v

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10:23 min

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July 31, 2014

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,
1Virginia Tech

Chapters

  • 00:05Title
  • 01:22Sample Preparation
  • 03:32Perfusion Experiment
  • 08:01Results: Glutamine Sensors Detect Transporter Activities in a Single Cell
  • 09:53Conclusion

Summary

Automatic Translation

Automatic Translation

This article will demonstrate how to monitor glutamine dynamics in live cells using FRET. Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. Experimental design, technical details of the experimental settings, and considerations for post-experimental analyses will be discussed for genetically encoded glutamine sensors.

Tags

Keywords: Genetically Encoded SensorsBiological MoleculesSubcellular ResolutionCellular Transport ProcessesTransporter DynamicsGlutamine SensorExperimental DesignTechnical DetailsPost-experimental Analyses

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