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September 06, 2014
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A method for rapid isolation of mitochondria from mammalian tissue biopsies is described. Rat liver or skeletal muscle preparations were homogenized with a commercial tissue dissociator and mitochondria were isolated by differential filtration through nylon mesh filters. Mitochondrial isolation time is <30 min compared to 60 - 100 min using alternative methods.
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Cite this Article
Preble, J. M., Pacak, C. A., Kondo, H., MacKay, A. A., Cowan, D. B., McCully, J. D. Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration. J. Vis. Exp. (91), e51682, doi:10.3791/51682 (2014).
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