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Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration

Article DOI: 10.3791/51682-v • 10:35 min • September 6th, 2014
September 6th, 2014 •
1, 2, 1, 2, 2, 1
1Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, 2Department of Anesthesiology, Perioperative and Pain Medicine, Boston Children's Hospital and Department of Anesthesia, Harvard Medical School

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Summary
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A method for rapid isolation of mitochondria from mammalian tissue biopsies is described. Rat liver or skeletal muscle preparations were homogenized with a commercial tissue dissociator and mitochondria were isolated by differential filtration through nylon mesh filters. Mitochondrial isolation time is <30 min compared to 60 - 100 min using alternative methods.

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Rapid Isolation Purification Mitochondria Transplantation Tissue Dissociation Differential Filtration Centrifugation Ficoll Gradient Centrifugation Commercial Tissue Dissociator Homogenization Nylon Mesh Filters Viable Mitochondria Respiration Competent Mitochondria
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