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In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
JoVE Journal
Biology
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JoVE Journal Biology
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

DOI:
10.3791/51683-v

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12:54 min

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September 04, 2014

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, ,
1Department of Life Science and Facility for Imaging by Light Microscopy,Imperial College London, 2Department of Life Sciences,Imperial College London

Chapters

  • 00:05Title
  • 01:52Labeling and Injection of HSPCs
  • 03:21Preparing the Mouse for Imaging
  • 05:20In Vivo Imaging: High-resolution Stacks and Time-lapse Acquisition
  • 11:25Results: In Vivo 4D Imaging of HSPCs in Mouse Calvarium
  • 12:16Conclusion

Summary

Automatic Translation

Automatic Translation

The nature of the interactions between hematopoietic stem and progenitor cells (HSPCs) and bone marrow niches is poorly understood. Custom hardware modifications and a multi-step acquisition protocol allow the use of two-photon and confocal microscopy to image ex vivo labeled HSPCs homed within bone marrow areas, tracking interactions and movement.

Tags

Keywords: Hematopoietic Stem CellsHSCsHematopoietic Stem And Progenitor CellsHSPCsBone Marrow MicroenvironmentIn Vivo Imaging4-dimensional TrackingConfocal ImagingTwo-photon ImagingOsteoblastic Cells

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