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A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

DOI:
10.3791/51693-v

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07:42 min

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June 05, 2014

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1Bacteriology and Parasitology,Tulane National Primate Research Center, 2Microbiology and Immunology,Tulane National Primate Research Center

Chapters

  • 00:05Title
  • 01:33Create Reference Image, Aligning Tissue for Dissected, and Annotate Slide
  • 03:29Load Consumable Mill Bit
  • 04:20Dissecting Tissue
  • 04:59Emptying and Removing Consumable Mill Bit
  • 05:32Results: Granulomas Caused by Mycobacterium tuberculosis can be Microdissected for RNA Extraction
  • 06:58Conclusion

Summary

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Microdissection has been extensively employed for the examination of DNA, RNA, and protein within tissue. Laser capture microscopy (LCM) is the most commonly used method, but a new milling technique, mesodissection, is recently available. We demonstrate RNA extraction from mesodissected formalin fixed paraffin embedded tissue slides of Mycobacterium tuberculosis granulomas.

Tags

Keywords: MicrodissectionLaser Capture MicroscopyMesodissectionMESO-1/MeSectr SystemHematoxylin And Eosin (H&E) StainingMycobacterium Tuberculosis (Mtb)GranulomaRNA Extraction

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